Anti Fungal Property of Calabash, Crescentia Cujete, Fruit in Bread Molds
Fernando Rampola Jr. | Hanissa Baraguir
Discipline: Education
Abstract:
Fungal growth is the most frequent cause of spoilage in bread products. To prevent economic losses
associated with spoilage, producers or the bread industry is now looking for alternatives that are plant
derived sources to the currently used preservatives. This study evaluated the antifungal property of
the calabash fruit Crescentia cujete extract at 100, 75 and 50%concentration on the growth of fungi
causing bread spoilage. Complete Randomized Design was used in three petri plates containing a
pure culture of bread molds that was subjected to 100, 75 and 50% concentrations of C. cujete extract,
and acetic acid as the control employing the Kirby-Bauer Disc Diffusion technique. There was a
significant difference (P<0.05) in the antifungal property of C. cujete extract (100, 75 and 50%) and
the acetic acid on the growth of fungi in bread. However, there were no significant differences
(P>0.05) between these three concentrations. The fruit extract of C. cujete at various concentrations
and control treatments against bread molds showed weak antifungal activity. Although the fruit extract
of the C.cujete showed weak antifungal activity, this implies that it has a potential to be developed as
an antifungal agent against bread molds. Further study is necessary to determine the optimum
concentration of the fruit extract that can inhibit the fungal growth in bread products.
References:
- ARBONNIER M. 2004. Trees, Shrubs and Liannas of West Africa Dry Zones, The Netherlands:ISBN MARGRAF PUBLISHERS GMBH. 191p.
- BILLACURA MP, PANGCOPA KKJ, 2017. Phytochemical screening, cytotoxicity, mutagenicity, antimutagenicity, and protective potentials of the different solvent extracts from the airdried leaves of Crescentia cujete Linn. International Journal of Advanced and Applied Sciences. 4(4):118-126.
- BLUMA RV, AMAIDEN MR, DAGHERO J, ETCHEVERRY M. 2008. Control of Aspergillus section Flavi growth and aflatoxin accumulation by plant essential oils. Journal of Applied Microbiology, 105: 203–214.
- BURKILL HM. 1985. The useful plants of West tropical Africa, 2nd ed. Royal Botanic Gardens, Kew, UK
- CANKAYA IIT, SOMUNCUOGLU EI. 2021. Potential and Prophylactic Use of Plants Containing Saponin-Type Compounds as Antibiofilm Agents against Respiratory Tract Infections. Evid Based Complement Alternat Med. doi: 10.1155/2021/6814215
- CATER SJ 1979. Tutorial Pharmacy. 6th Edn. Pitman Medical Publishing Co. Ltd. Tunbridge Well kent, England. pp. 29-30 Eva R (1983). “Food, Health and You”. A Book on Nutrition with Special Reference to East Africa. Macmillan Publishers, London. pp.14-25.
- CAUVIAN, SP 2003. Bread Making, Improving Quality, 1 st ed. Wood Head Publishing Limited: Elsevier pp. 589.
- CHATTOPADHYAY RR, BHATTACHARYYA SK. 2007. Terminalia chebula: An Update. Pharmacognosy Reviews, 1(1): 151-156.
- DAL BELLO F, CLARKE CI, RYAN LAM, ULMER H, SCHOBER TJ, STRŐM K, SIŐGREN J, VAN SINDEREN D, SCHNÜRER J, ARENDT EK. 2007. Improvement of the quality and shelf life of wheat bread by fermentation with the antifungal strain Lactobacillus plantarum FST 1.7. Journal of Cereal Science, 45: 309–318.
- EJELONU BC, OLUWAFEMI AD, LASISI AA, OLAREMU AG, EJELONU OC. 2011. Chemical constituents of calabash (Crescentia cujete). African Journal of Biotechnology, 10(84): 19631-19636.
- GUTIERREZ LC, SANCHEZ R, BATLLE R, NERIN C. 2009. New antimicrobial active package for bakery products. Trends in Food Science & Technology, 20 (2): 92-99.
- GUYNOT ME, RAMOS AJ, SALA D, SANCHIS V, MARIN S. 2002. Combined effects of weak acid preservatives, pH and water activity on growth of Eurotium species on a sponge cake. International Journal of Food Microbiology, 76 (1-2): 39- 46
- LEGAN JD, 1993. Mould spoilage of bread: the problem and some solutions. International Biodeterioration & Biodegradation, 32(1-3): 33–53
- MAGNO IT, ESMAIL RP, LIM MSS, PADUA JNJD, REYES KL, TUGOM LAG, VALDEZ AG, ABUSAMA HP. 2021. Antibacterial Effect of Calabash (Crescentia cujete) leaf and Fruit extract on Preservation of Lettuce (Lactuca sativa) leaves with Escherichia coli. Asean Journal of Science and Engineering, 2(1): 91-94.
- MAHBUB KR, HOQ Md.M, AHMED MM. AND A. SARKER A. 2011. In vitro antibacterial activity of Crescentia cujete and Moringa oleifera. Bangladesh Research Publications Journal. 5 (4):337-343
- MORTON JM. 1968. The Calabash ( Crescentia cujete) in Folk Medicine. New York: Economic Botany, 22(3): 273–280.
- MORTON JM. 1981. Atlas of medicinal plants of Middle America: Bahamas to Yucatan. Springfield, III, (USA) C.C. Thomas
- NIELSEN PV, RIOS R. 2000. Inhibition of fungal growth on bread by volatile components from spices and herbs, and the possible application in active packaging, with special emphasis on mustard essential oil. International Journal of Food Microbiology, 60(2-3): 219–229.
- PARVIN MS, DAS N, AND JAHAN N. 2015. Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark. BMC Research Notes 8(412).
- SAHAN Y, 2011. Effect of Prunus laurocerasus L. (Cherry Laurel) leaf extracts on growth of bread spoilage fungi. Bulgarian Journal of Agricultural Science, 17(1): 83-92. SEGISMUNDO AB, FLORENDO PE, PABLICO AR. 2008. In vitro antifungal activity of Gouania javanica Miq. Leaves. UNP Research Journal, 17:1-10.
- VIACAVA GE, ANSORENA MR, ROURA SI, AGUILAR GAG, AYALA-ZAVALA JF 2013. Fruit Processing Byproducts as a source of Natural Antifungal Compounds. Antifungal Metaabolites from plants, Springer-Verlag Berlin Heidelberg, 447-461.