Home⇛Health Sciences Journal⇛vol. 11 no. 2 (2022)

Determination of the efficacy of okra seed powder in aqueous solution as a glucose lowering agent compared to acarbose in STZ diabetic rats

Milagros B. Rabe | Ma. Peñafrancia L. Adversario | Joseph C. Chua | Melissa Marie R. Rondina | Jennifer M. Nailes | Venus A. Solar

 

Abstract:

Introduction Okra is reported to have anti-diabetic effects, but the literature shows conflicting results. The experiment aimed to determine the efficacy of three doses of okra seed powder suspension as a glucose lowering agent on streptozotocin-induced diabetic rats and its cellular effects on the liver and pancreas. Methods Twenty-five Sprague Dawley rats that were given streptozotocin 60 mg/kg intraperitoneally were randomly allocated to one of five treatment groups: okra seed powder at 100 mg/kg, 150 mg/kg and 200 mg/kg, acarbose (positive control) and vehicle only (negative control). The treatments were given as a 1.5 mL oral gavage daily for 21 days. Significant differences in blood glucose were determined between treatment groups in terms of relative change from baseline, using One-Way ANOVA with Dunnett’s method with acarbose as the referent group. Repeated measures ANOVA was used to analyze the blood glucose levels across the time point collections (baseline, T1 and T2). Histopathologic changes on the liver and pancreas were described using counts and proportions. Results Mean blood glucose values increased from baseline to T2 in all treatment groups. Increasing trend was observed only up to T1 in the 150 mg/kg and the 200 mg/kg okra seed treatment groups. Comparing okra treatment groups to acarbose, the percentage increase of mean blood glucose from baseline to T2 was lowest in the 200 mg/kg okra group (p = 0.040). The okra-treated rats had no fatty change and a dose-dependent decrease in cellular degeneration in the liver and none for the 200 mg/ kg treatment group. Conclusion The 200 mg/kg okra suspension has a potential lowering effect on blood glucose and a hepatoprotective effect. A longer period of observation with higher doses of okra suspension is recommended to study these effects further.



References:

  1. American Diabetes Association. Classification and Diagnosis of Diabetes: Standards of Medical Care 2021. Diabetes Care [Internet]. 2021; 44(Suppl. 1): S15–S33. Available from: https://doi.org/10.2337/dc21-S00
  2. Wo r l d H e a l t h O r g a n i z a t i o n . G l o b a l Re p o r t o n Diabetes [Inter net]. 2016. Retrieved on June 20, 2 0 1 8 . A v a i l a b l e f r o m : a p p s . w h o . i n t / i r i s /bitstream/10665/204871/1/9789241565257eng.pdf
  3. Philippine Statistics Authority. Deaths in the Philippines, 2016 [internet]. Philippine Statistics Authority. Retrieved from: https://psa.gov.ph › People › Vital Statistics on July 20, 2018.
  4. World Health Organization. Transforming our World: The 2030 Agenda for Sustainable Development. World Health Organization [Internet]. 2015. Retrieved on June 8, 2022. Available from: https://sdgsun.org/publications/transforming-our-world-2030-agenda-sustainable-development-17981
  5. Riddle MC, Herman WH. The cost of diabetes care — an elephant in the room. Diabetes Care [Internet]. 2018 May; 41(5): 929-32. Retrieved from: https://doi.org/10.2337/dci18-0012
  6. Paz-Pacheco E, Jimeno C. Diabetes care in the Philippines. Journal of the ASEAN Federation of Endocrine Societies [Internet]. 2015 Nov; accessed: 29 July 2018; 30(2): 118. Available from: http://asean-endocrinejournal.org/index.php/JAFES/article/view/267/667.
  7. Tan GH. Diabetes care in the Philippines. Ann Global Health [Internet]. 2016; 81(6): 863–9. doi: http://doi.org/10.1016/j.aogh.2015.10.004
  8. Zhang L, Chen Q, Li L, et al. Alpha-glucosidase inhibitors and hepatotoxicity in Type 2 diabetes: A systematic review and meta-analysis. Sci Rep [Internet]. 2016 Sep 6; 6: 32649. doi: 10.1038/srep32649
  9. Chuang CY, Hsu C, Chao CY, Wein YS, Kuo YH, Huang CJ. Fractionation and identification of 9c, 11t, 13t-conjugated linolenic acid as an activator of PPARalpha in bitter gourd (Momordica charantia L.). J Biomed Sci [Internet]. 2006 Nov; 13(6): 763-72. doi: 10.1007/s11373-006-9109-3
  10. Sasa M, Komoda T, Awata T, Katayama S. Activating effect of momordin, extract of bitter melon (Momordica charantia L.), on the promoter of human PPARdelta. J Atheroscler Thromb [Internet]. 2009; 16(6): 888-92. doi: 10.5551/jat.2790
  11. Ülger TG, Çakiroglu FP. The effects of onion (Allium cepa L.) dried by different heat treatments on plasma lipid profile and fasting blood glucose level in diabetic rats. Avicenna J Phytomed [Internet]. 2020 Jul-Aug; 10(4): 325-33.
  12. Nazarian-Samani, Z, Sewell RDE, Lorigooini Z, et al. Medicinal plants with multiple effects on diabetes mellitus and its complications: A systematic review. Curr Diab Rep [Internet]. 2018; 18: 72. Available from: https://doi.org/10.1007/s11892-018-1042-0
  13. Bontilao ED, Digman KM, Fernandez EL, et al. The effect of 100% Abelmoschus esculentus Linn. (okra) fruit extract on alloxan-induced hyperglycemic male Mus musculus (mice). 2007. Thesis. [Abstract] Retrieved from Herdin Record #: R07-CDU-13071713492197
  14. Sabitha V, Ramachandran S, Naveen KR, Panneerselvam K. Antidiabetic and antihyperlipidemic potential of Abelmoschus esculentus (L.) Moench in streptozotocin-induced diabetic rats. J Pharm Bioallied Sci [Internet]. 2011 Jul-Sep; 3(3): 397–402. doi: 10.4103/0975-7406.84447
  15. Gemede HF, Ratta N, Haki GD, Woldegiorgis AZ, Beyene F. Nutritional quality and health benefits of okra (Abelmoschus esculentus): A review. J Food Process Technol [Internet]. 2015; 6: 458. doi:10.4172/2157-7110.100045
  16. Shahreza, FD. Hibiscus esculentus and diabetes mellitus. J Nephropharmacol [Internet]. 2016; 5(2): 104–5. Retrieved from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297561/
  17. Thanakosai W, Phuwapraisirisan P. First identification of α-glucosidase inhibitors from okra (Abelmoschus esculentus) seeds. Nat Prod Commun [Internet]. 2013 Aug; 8(8): 1085-8.
  18. Almeda CND, Aludo RGC, Azcona JMA, et al. A comparative study on the effectiveness of Momordica charantia (ampalaya) leaves decoction versus pounded Hibiscus esculentus (okra) seed tea in lowering capillary blood glucose levels among selected Type II diabetic patients in Cebu City. 2010. Thesis. [Abstract] Retrieved from Herdin Record #: R07-CDU-13020115022716
  19. Alingod A, Allam JC, Gordon K, Macatuggal JP, Ramos J, Vergara J. Slipping through diabetes: Unshrouding the hypoglycemic capabilities of okra (Abelmoschus esculentus). Thesis. St. Louis University School of Health Sciences Bulletin vol 1. June 2012-March 2015. [Abstract] Retrieved from Herdin Record #: R02-USL-16052613365962
  20. Magbitang MA, Samaniego IM. Randomized open-label study comparing the effect of Abelmoschus esculentus water vs placebo in postprandial blood glucose on normal human subjects. The Filipino Family Physician. 2016 Oct-Dec; 54(4): 151-9. [Abstract] retrieved from Herdin Record #: PCHRD17020608395439
  21. Arifin WN, Zahiruddin WM. Sample size calculation in animal studies using resource equation approach. Malays J Med Sci [Internet]. 2017 Oct; 24(5): 101-5. doi:10.21315/mjms2017.24.5.11
  22. Furman BL. Streptozotocin-induced diabetic models in mice and rats. Current Protocols [Internet]. 2021; 1: e78. doi: 10.1002/cpz1.78
  23. Johnson PD, Besselsen DG. Practical aspects of experimental design in animal research. ILAR Journal [Internet]. 2022; 34(4): 202-6. Retrieved from https://doi.org/10.1093/ilar.43.4.202
  24. Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm [Internet]. 2016 Mar; 7(2): 27-31. doi:10.4103/0976-0105.177703
  25. Johns Hopkins University Animal Care and Use Committee. Policies and guidelines. Available from: http://web.jhu.edu/animalcare/procedures/rat.html#anesthesia
  26. Acarbose (Glucobay Monograph). Retrieved from: https://www.bayer.ca/omr/online/glucobay-pm-en.pdf
  27. Drugs.com (n.d.). Acarbose [Drug Information]. https://www.drugs.com/monograph/acarbose.html
  28. University of California - Davis. IACUC Guide 31. Blood collection: Volumes, frequencies and sites. [Internet]. 2019. Retrieved from https://research.ucdavis.edu/wp-content/uploads/IACUC-31.pdf
  29. National Institutes of Health - Office of Animal Care Use. Guidelines for Euthanasia of Rodents Using Carbon Dioxide [Internet]. 2019 Retrieved from https://oacu.oir.nih.gov/system/files/media/file/2021-06/b5_euthanasia_of_rodents_using_carbon_dioxide.pdf
  30. Honjo K, Doi K, Doi C, Mitsuoka T. Histopathology of streptozotocin-induced diabetic DBA/2N and CD-1 mice. Lab Anim [Internet]. 1986 Oct; 20(4): 298-303. doi:10.1258/002367786780808695
  31. Junod A, Lambert AE, Stauffacher W, Renold AE. Diabetogenic action of streptozotocin: Relationship of dose to metabolic response. J Clin Investigation [Internet]. 1969; (48): 2129-39. Retrieved from: https://dm5migu4zj3pb. c l o u d f r o n t . n e t / m a n u s c r i p t s / 1 0 6 0 0 0 / 1 0 6 1 8 0 / JCI69106180.pdf
  32. Wang Z, Yang Y, Xiang X, Zhu Y, Men J, He M. Estimation of the normal range of blood glucose in rats. Wei Sheng Yan Jiu [Internet]. 2010 Mar; 39(2): 133-7, 142. Chinese. PMID: 20459020. [Translated Abstract] Retrieved from: https://pubmed.ncbi.nlm.nih.gov/20459020/
  33. Rendell M. The role of sulphonylureas in the management of Type 2 diabetes mellitus. Drugs [Internet]. 2004; 64(12): 1339-58. doi: 10.2165/00003495-200464120-00006
  34. Alqasoumi SI. ‘Okra’ Hibiscus esculentus L.: A study of its hepatoprotective activity. Saudi Pharm J [Internet]. 2012 Apr; 20(2): 135-41. doi: 10.1016/j.jsps.2011.10.002
  35. Wahyuningsih SPA, Sajidah ES, Atika BND,Winarni D, Pramudya M. Hepatoprotective activity of okra (Abelmoschus esculentus L.) in sodium nitrite-induced hepatotoxicity. Veterinary World [Internet]. 2020; 13(9): 1815-21.
  36. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res [Internet]. 2001; 50(6): 537-46.

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