Home⇛Science Asia Review⇛vol. 10 no. 1 (2024)

Turmeric (CURCUMA LONGA LINN.) Rhizome Extract: Its Antiinflammatory Effect Using CHORIOALLANTOIC MEMBRANE (CAM) Assay

Nelsa Maradul

Discipline: Pharmacology

 

Abstract:

Turmeric (Curcuma longa Linn.) is a herbaceous plant member of the ginger family (Zingiberaceae) which is widely known in the Philippines for its uses in culinary as a spice and scientific world as a herbal plants. Turmeric is scientifically proven to have antioxidant, antiinflammatory, and anti-cancer effect.Turmeric has a lot benefit when it comes to health, as it can help in the management of inflammatory conditions arthritis, anxiety, and hyperlipidemia. It can also help in management of exercise induced inflammation and muscle soreness, thus enhancing recovery and subsequent performance in active people.The study aimed to determine the anti-inflammatory effect of Turmeric (Curcuma longa Linn.) by Chorioallantoic Membrane Assay. The Chorioallantoic Membrane Assay is method that uses duck embryo through incubation process. The study was experimentally conducted using various procedures from phytochemical screening of the plants, preparation of the materials and plant samples, incubation of duck egg, ethanolic extraction, and CAM Assay. The anti-inflammatory effect of two test solutions, 100% and 75% concentration showed significant difference wherein 100% concentration of test solution resulted in a commutative score of 5.67 which is interpreted as having moderate irritation. While the 75% concentration of test solution has a commutative score of 3.90 which interpreted as slight irritation. Therefore, 75% concentration of test solution has lesser changes in irritation on CAM Assay test. The commercially available drug Diclofenac sodium 25mg/1mL with the mean of 0.80 interpreted as partially none. the findings revealed, the 75% concentration and Diclofenac sodium are statistically high significant.



References:

  1. Aggarwal, B. B., Sundaram, C., Malani, N., & Ichikawa, H. (2013). Curcumin-free turmeric exhibits anti-inflammatory and anticancer activities: Identification of novel components of turmeric. Retrieved from https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=turmeric+as+Antiinflammatory+related+stufies&btnG=#d=gs_qabs&t=1667910726650&u=%23p%3DXwoF7VaLAGcJ
  2. Aceda, J. Z., Reyes, C. T., & Rodriguez, E. B. (2012). Chemical composition of purslane (*Portulaca oleracea* L.) and anti-inflammatory activity of its lipid components. Retrieved from https://www.ukdr.uplb.edu.ph/journalarticle/4731
  3. Balls, M., Blaauboer, B., Brusick, D., Frazier, J., Lamb, D., Poncelet, J., ... & Spielmann, H. (1995). ICCVAM-recommended test method protocol: Hen’s Egg Test – Chorioallantoic Membrane (HET-CAM) Test Method. Retrieved from https://ntp.niehs.nih.gov/sites/default/files/iccvam/docs/protocols/ivocular-hetcam
  4. Boarescu, L., Boarescu, P.-M., & Bocsan, R. C. (2021). Curcumin nanoparticles enhance antioxidant efficacy of diclofenac sodium in experimental acute inflammation. Retrieved from https://www.mdpi.com/2227-9059/10/1/611
  5. Dragland, I. S. (2013). HET-CAM test. Retrieved from https://niom.no/het-camtest/HET%2DCAM%20test%20(hen's%20egg,membrane%20(CAM)%20is%20exposeD
  6. Hewlings, S. J., & Kalman, D. S. (2017). Curcumin: A review of its effects on human health. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664031/
  7. Jain, S. K. (2018). Medicinal plants. Retrieved from https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=medicinal+plants+related+studies&btnG=#d=gs_qabs&t=1667913428890&u=%23p%3DFN8gXeGD_tgJ
  8. Jumdail, R., Tugbo, K., & Sablay, E. (2018). Anti-inflammatory of *Cadena de Amor* (*Antigonon leptopus*) leaf extract by chorioallantoic membrane assay (CAM).
  9. Kancherla, N., Dhakshinamoothi, A., Chitra, K., & Komaran, R. B. (2019). Preliminary analysis of phytoconstituents and evaluation of anthelmintic property of *Cayratia auriculata* (In Vivo). Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035446/
  10. Laguipo, A. B. B. (2016). Health benefits of turmeric. Retrieved from https://www.news-medical.net/health/Health-Benefits-of-Turmeric.aspx
  11. Medzhitov, R. (2016). Origin and physiological role of inflammation. Retrieved from https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=types+of+inflammation+related+studies&btnG=#d=gs_qabs&t=1667912593692&u=%23p%3DJ12En9KhgHMJ
  12. Prasad, S., & Aggarwal, B. B. (2011). Turmeric, the golden spice. Retrieved from https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=turmeric+as+medicinal+plant&oq=Turmeric+as+medi#d=gs_qabs&t=1667915694961&u=%23p%3DN-l5ErduluUJ
  13. Ribatti, D. (2016). The chick embryo chorioallantoic membrane (CAM) assay. Retrieved from https://pubmed.ncbi.nlm.nih.gov/27832950/
  14. Schmid-Schonbein, G. W. (2016). Analysis of inflammation. Retrieved from https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=inflammation+related+studies&oq=inflammation+related+st#d=gs_qabs&t=1667912043125&u=%23p%3DHrFEi9uEefsJ
  15. Stuart, G. U. (2017). Dilaw. Retrieved from http://www.stuartxchangePh.com/Dilaw.html
  16. Verma, S., Jain, S., & Prasad, S. (2018). Medicinal properties of turmeric (*Curcuma longa* L.): A review. Retrieved from https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=turmeric+as+medicinal+plant&oq=Turmeric+as+medi#d=gs_qabs&t=1667915699753&u=%23p%3DcgGg7ZS_cxEJ.
  17. Ying, Y., Zhang, Y., & Guo, Y. (2021). Anti-inflammatory effects of curcumin in inflammatory diseases: Status, limitations, and countermeasures. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572027/#:~:text=Clinical%20trials%20focused%20on%20the,and%20oxidative%20stress%20%5B60%5D

Tools


Copyright © 2025  KITE Digital Educational Solutions |  Exclusively distributed by CE-Logic