HomeJournal of Interdisciplinary Perspectivesvol. 3 no. 4 (2025)

Development and Sensory Evaluation of Herbal Tea Products from Leaves and Fruits of Mulberry (Morus alba)

Glennadi R. Rualo | Rose F. Cortez | Crystal Monique J. Estipular | Michelle V. Reyes | Jervin S. Aquino

Discipline: Food and Beverage Production

 

Abstract:

This study explores the development and evaluation of two innovative herbal beverages: mulberry tea and mulberry-flavored kombucha tea. Leveraging the therapeutic potential of mulberry leaves and fruits (Morus alba), the research aims to create affordable, nutritious, and consumer-accepted alternatives to conventional beverages. Mulberry is renowned for its health-promoting properties, including antioxidant, antidiabetic, and anti-inflammatory effects, while kombucha, a fermented tea, is celebrated for its probiotic and detoxifying benefits. An experimental research design assessed the beverages' sensory, microbial, and nutritional properties. Mulberry tea was prepared using sun-drying and air-drying methods, with air-dried samples showing superior sensory attributes. Kombucha was prepared by fermenting mulberry tea with a symbiotic culture of bacteria and yeast (SCOBY) over two fermentation phases. An analysis of sensory evaluations from 100 respondents, using the Mann-Whitney U test, highlighted the acceptability of air-dried mulberry tea and kombucha prepared with 10 tea bags, achieving a more balanced flavor profile than other formulations. Microbial analysis confirmed the safety of the beverages, with no detection of harmful bacteria such as Salmonella and Escherichia coli. Nutritional analysis revealed that the kombucha samples contained essential macronutrients and were free from fats and cholesterol. Sample 1 offered higher protein content and caloric value, making it suitable for energy-conscious consumers. The results demonstrated that mulberry and kombucha tea are viable health-oriented beverages with significant commercial potential. Both products received high overall acceptability scores, showcasing their ability to meet consumer preferences. The study underscores the potential of mulberry-based beverages to align with the growing global demand for functional and wellness-oriented drinks. In conclusion, this research highlights the versatility and economic value of mulberry leaves and fruits, paving the way for innovative, health-promoting beverage options suitable for large-scale production.



References:

  1. Acharya, R., Bagchi, T., & Gangopadhyay, D. (2022). Mulberry as a valuable resource for food and pharmaceutical industries: A review. In S. Kumar (Ed.), Medicinal Plants. IntechOpen. https://doi.org/10.5772/intechopen.104631
  2. Al-Mohammadi, A.-R., Ismaiel, A.A., Ibrahim, R.A., Moustafa, A.H., Abou Zeid, A., & Enan, G. (2021). Chemical constitution and antimicrobial activity of kombucha fermented beverage. Molecules, 26(16), 5026. https://doi.org/10.3390/molecules26165026
  3. Chandrasekara, A., & Shahidi, F. (2018). Herbal beverages: Bioactive compounds and their role in disease risk reduction - A Review. Journal of Traditional and Complementary Medicine, 8(4), 451–458. https://doi.org/10.1016/j.jtcme.2017.08.006
  4. Civille, G. V., Carr, B. T., Osdoba, K. E. (2024). Sensory Evaluation Techniques. CRC Press. https://doi.org/10.1201/9781003352082
  5. De Leon, Jovi (2023, June 15). Pampanga home to PH’s 1st mulberry tea factory. Sunstar. Retrieved from https://tinyurl.com/247uad67
  6. De Miranda, J. F., Ruiz, L. F., Silva, C. B., Uekane, T. M., Silva, K. A., Gonzalez, A. G. M., Fernandes, F. F., & Lima, A. R. (2022). Kombucha: A review of substrates, regulations, composition, and biological properties. Journal of Food Science, 87(2), 503–527. https://doi.org/10.1111/1750-3841.16029
  7. Dubey, K. K., Janve, M., Ray, A., & Singhal, R. S. (2020). Ready-to-drink tea. In Trends in Non-alcoholic Beverages (pp. 101–140). Elsevier. https://doi.org/10.1016/B978-0-12-816938-4.00004-5
  8. Freitas, A. (2020). The Ultimate Kombucha Guide. Mushroom Momma. Retrieved from https://tinyurl.com/3tmaaa7r
  9. Gilbert, R. J., de Louvois, J., Donovan, T., Little, C., Nye, K., Ribeiro, C. D., Richards, J., Roberts, D., & Bolton, F. J. (2000). Guidelines for the microbiological quality of some ready-to-eat foods sampled at the point of sale. PHLS Advisory Committee for Food and Dairy Products. Communicable Disease and Public Health, 3(3), 163–167. https://pubmed.ncbi.nlm.nih.gov/11014026/
  10. Goh, W.N., Rosma, A., Kaur, B., Fazilah, A., Karim, A.A., & Bhat, R. (2012). Fermentation of black tea broth (Kombucha): I. Effects of sucrose concentration and fermentation time on the yield of microbial cellulose. International Food Research Journal, 19(1), 109–117. https://tinyurl.com/a6h6ta93
  11. Greenwalt, C.J., Steinkraus, K.H., & Ledford, R.A. (2000). Kombucha, the fermented tea: Microbiology, composition, and claimed health effects. Journal of Food Protection, 63(7), 976–981. https://doi:10.4315/0362-028x-63.7.976
  12. Jnani, D., & Ray, S. D. (2024). Escherichia coli. In: P. Wexler (Ed), Encyclopedia of Toxicology (Fourth Edition (pp 357-367). Oxford: Academic Press. https://doi:10.1016/b978-0-12-824315-2.00190-1
  13. Kitwetcharoen, H., Phung, L.T., Klanrit, P., Thanonkeo, S., Tippayawat, P., Yamada, M., & Thanonkeo, P. (2023). Kombucha healthy drink—recent advances in production, chemical composition and health benefits. Fermentation, 9(1), 48. https://doi.org/10.3390/fermentation9010048
  14. Larasati, A., & Issutarti. (2017). The analysis of consumer preferences on mulberry leaves tea as an antioxidant-enriched product. In: M. Munizu, M.Y. Zamhuri, R. Shunsuke, G. Pagalung, M. Alexander, S. Chamberlain, M. Nohong (Eds.), Proceedings of the 2nd International Conference on Accounting, Management, and Economics (ICAME), Makassar, Indonesia, 41-51.
  15. Malbaša, R.V., Lončar, E.S., Vitas, J.S., & Čanadanović-Brunet, J.M. (2011). Influence of starter cultures on the antioxidant activity of kombucha beverage. Food Chemistry, 127(4), 1727–1731. https://doi.org/10.1016/j.foodchem.2011.02.048
  16. Martinez L.J., Valenzuela Suárez, L., Jayabalan, R., Huerta Oros, J., & Escalante-Aburto, A. (2018). A review on health benefits of kombucha nutritional compounds and metabolites. CyTA– Journal of Food, 16(1), 390–399. https://doi.org/10.1080/19476337.2017.1410499
  17. Pastore R.L., & Fratellone P. (2006) Potential health benefits of green tea (Camellia sinensis): a narrative review. Explore, 2(6), 531-539. https://doi.org/10.1016/j.explore.2006.08.008
  18. Piljac-Žegarac, J., Šamec, D., & Piljac, A. (2013). Herbal teas: A focus on antioxidant properties. In V. R. Preedy (Ed.), Tea in health and disease prevention (pp. 129–140). USA. Academic Press. https://doi.org/10.1016/B978-0-12-384937-3.00011-2
  19. Raja, K.S., Taip, F.S., Azmi, M.M., & Shishir, M. R. (2019). Effect of pre-treatment and different drying methods on the physicochemical properties of Carica papaya L. leaf Powder. Journal of the Saudi Society of Agricultural Sciences, 18(2), 150–156. https://doi.org/10.1016/j.jssas.2017.04.001
  20. Richards, L. (2020). 2,000 calorie diet: Is it healthful? Retrieved from https://www.medicalnewstoday.com/articles/2000-calorie-diet
  21. Sanchez, M. D. (2000). Mulberry: An exceptional forage available almost worldwide! Retrieved from https://tinyurl.com/2extvx6t
  22. Streit, L. (2021). What are macronutrients? all you need to know. Retrieved from https://tinyurl.com/mrv779r3
  23. Villarreal-Soto, S.A., Beaufort, S., Bouajila, J., Souchard, J.-P. and Taillandier, P. (2018), Understanding kombucha tea fermentation: A Review. Journal of Food Science, 83, 580-588. https://doi.org/10.1111/1750-3841.14068
  24. Yigit, D., Akar, F., Baydas, A., & Buyukyildiz, M. (2010). Elemental composition of various mulberry species. Asian Journal of Chemistry, 22, 3554–3560