Home⇛Scientific Augustinian⇛vol. 13 no. 1 (2023)

Evaluation of Cytotoxicity and Antibacterial Activity of Pili (Canarium ovatum) Pericarp Aqueous Fraction Against Methicillin-Resistant Staphylococcus aureus (ATCC BAA-1708)

Eric Justin Blancaflor | Allan Marc Lena | Jenny Marie Blancaflor | Irish Cordero | Uvrie Dell Bawe | Janna Pearl Benliro | Nicolin Kwin Cardinal | Karen Carranza | Kyla Ann Marie Coling | Kyla Dayot | Reiiel Gabrielle Dela Gente | Shan Francine Depositario | Deiana Louise Fernandez | Jules Albert Juanito | Mary Grace Lamis | Christine Kate Luces

Discipline: Toxicology

 

Abstract:

Methicillin-resistant Staphylococcus aureus poses a significant public health threat. Pili (Canarium ovatum) family Burseraceae, a plant native to the Philippines, particularly in Sorsogon City, Bicol Region, has long been used in traditional medicine. Recent studies indicate that the pericarp of Pili contains bioactive compounds with antibacterial and cytotoxic properties. This study aimed to assess the antibacterial and cytotoxic activities of the aqueous fraction of Pili pericarp. The antibacterial activity of the aqueous fraction was evaluated using microbroth susceptibility assay against methicillin resistant S. aureus (MRSA) ATCC BAA-1708 at a concentration range of 5-7.5 mg/mL. At the highest concentration (7.5 mg/mL), inhibition was only 17.87%, indicating that the aqueous fraction of Pili has limited antibacterial activity at the tested concentrations. Additionally, the cytotoxicity of the aqueous fraction was evaluated using the Brine Shrimp Lethality Assay (BSLA), with results showing that a concentration of 3 mg/mL remained safe after 24 hours of exposure. These findings suggest that while the aqueous fraction is relatively safe, its antibacterial activity is limited at the concentrations tested.



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