HomeAnnals of Tropical Researchvol. 38 no. 1 (2016)

Formulation And Testing Of Combined Organic Liquid Supplement From Trichoderma Spp. And Fermented Plant And Seed Extracts On The Growth Of Organic Pechay

Ellen S. Romero | Lani Lou Mar A. Lopez | Fe L. Porciuncula | Purisima P. Juico | Jonathan L. Galindez

 

Abstract:

The Ramon Magsaysay Center for Agricultural Resources and Environment Studies (RM-CARES) has isolated Trichoderma longibrachiatum and Trichoderma asperellum from carabao manure. Since Trichoderma is mass produced in solid form and applied basally, the conversion of Trichoderma into liquid form allows the supply of nutrients at different growth stages of crops. This study aimed to formulate and test the efficacy of combined organic liquid supplement (OLS) from Trichoderma spp. and fermented extracts from kakawate and malunggay leaves, banana fruits, soybean and mungbean seeds. Based on the results for one trial on pechay, undertaken in a certified organic area at the Ramon Magsaysay Center for Agricultural Resources (RM-CARES), Central Luzon State University Science City of Muñoz (CLSU), Nueva Ecija, yield was significantly increased by the supplementation of Trichoderma spp. in combination with fermented plant and seed extracts. Treatment with 1:0.5 ratio of T. asperellum : fermented plant and seed extracts significantly gave the highest computed yield of 12 t/ha which is 47% higher than the yield obtained in commercial OLS and 106%higher than the control. The treatment with 1:0.5 ratio of T. longibrachiatum : fermented plant and seed extracts, and treatment with commercial OLS produced comparable yield with 9.80 t/ha and 8.17 t/ha, respectively. Since the developed OLS from Trichoderma and fermented plant and seeds extracts is at par or even surpassed the yield of commercial OLS, it has the potential as bio-liquid fertilizer for organic pechay production which could be possibly substituted to commercial OLS.



References:

  1. ABADILLA, D.C. 1982. Organic Farming. Quezon City: AFA Publications, Inc.
  2. AWAD, F.K., W. KHALIL and A.H. MAKSOUD, 1993. Comparative effects of some organic manures and bentonite as soil amendments. Agrochemical 37:101-106.
  3. BENITEZ, T, A.M. RINCON, M.C. LIMON and A.C. CODON. 2004. Bio-control mechanisms of Trichoderma Strains. Int, Microbiolo, 7(4): 249-260.
  4. COOK, R. J and K. F. BAKER. 1983. The Nature and Practice of Biological Control of Plant Pathogens. APS Press.
  5. COOPERBAND, L. 2002. Building soil organic matter with organic ammendments. Center for Integrated Agriucltural Sciences (CIAS) College of Agricultural and Life Sciences University of Wisconsin-Madison.
  6. DELA CRUZ, N.E. M.G. PATRICIO, S.A. LINDAIN, and E.S. ROMERO. 2007. Utilization of organic fertilizer in intensive small scale organic vegetable production. Compilation of Technical Papers Presented. Agency In-house Review of Completed and On-going Research Projects. Research (Farm Resources). RET-CLSU, Science City of Muñoz, Nueva Ecija.
  7. HARMAN, G.E. 2000. Myths and dogmas of bio-control: Changes in perceptions derived from research on Trichoderma harzianum T22. Plant Disease. 84: 377-393.
  8. HARMAN, G. E., C.R., HOWELL, A. VITERBO, I. CHET, and M. LORITO. 2004. Trichoderma species opportunistic, a virulent plant symbionts. Nat. Rev. Microbiol. 2:43-56.
  9. JIRAPORNCHAREON, S. 1993. The use of chemical and organic fertilizers in crop production in Thailand. Food Fertilizer Technology Center (FFTC) for the ASPAC region. Extension Bulletin n° 370.
  10. KRAWCZYK, M. Make Your Own Liquid Garden Fertilizers www.keylinevermont.com.
  11. LOPEZ, L.L.M.A., C.P. AGANON and P.P JUICO. 2014. Isolation of Trichoderma species from carabao manure and evaluation of its beneficial uses. International Journal of Science and Technology. 3 (8): 2277-8616.
  12. LOPEZ, L.L.M.A.., R. G. REYES and D.G. ALVINDIA. 2015. Evaluation of two species of Trichoderma as compost activator and bio-control agents. Journal of Agricultural Technology 11(2):525-537
  13. MAHESHWARI, D. K and R.C. DULAY. 2008. Potential microorganisms for sustainable agriculture: A Techno-Commercial. I.K. International Publishing House Pvt. Ltd. S-25, Green Park Extension, Uphaar Cinema Market, New Delhi 110016 (India).
  14. PASCUAL, PET.R.L., A.D. JARWAR and P.S. NITURAL. 2013. Fertilizer, fermented activators and EM utilization in pechay (Brassica Pekinensis L.) production. Pak. J. Agri., Agril. Engg., Vet. Sci., 29 (1): 56-69.
  15. REPETTO R. and S.S. BALIGA. 1996. Pesticides and the Immune System: The Public Health Risks. Washington, DC:World Resources Institute.
  16. ROMHIYAT S. M., M. SURYA and P. B. HASTUTI. 2006. [Effect of sollubility and incubation time (with aeration) of organic material on chinese mustard (Brassica juncea)]. Buletin Ilmiah Instiper 13(1):1-11.
  17. SAMUELS, G. J. 2006. Trichoderma: Systematics, the sexual state, and ecology. Phytopathology 96:195-206.
  18. SOPHA, G.A and T.S.UHAN. 2013. Advances in Agriculture & Botanics. International Journal of the Bioflux Society 5(1).
  19. TANG, W., H. YANG, and M. RYDER. 2001. Research and application of Trichoderma spp. in biological control of plant pathogen. In: Bio-Exploitation of Filamentous Fungi (eds. S.B. Pointing and K.D. Hyde), Fungal diversity Research Series 6: 403-435.
  20. VINALE, F., R. MARRA, F. SCALA, E.L. GHISALBERT, M. LORITO, and K. SIVASITHANAMPARAM. 2006. Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Letters in Applied Microbiology 43: 143-148.