Home⇛Science and Technology Journal⇛vol. 4 no. 1 (2014)

Isolation, Characterization, and Evaluation of Plant Growth-Promoting Rhizobacteria from Rhizospheres of Sugarcane

Dionisio S. Bucao | Diana Rose De Leon

Discipline: Agriculture

 

Abstract:

The role of plant-growth promoting rhizobacteria (PGPR) in sustaining organic crop production system is indispensable. As such, this study characterized and evaluated the rhizobacteria isolated from the rhizospheres of wild lily sp. and sugarcane in the City of Batac, Ilocos Norte, Philippines. Initially, 15 bacterial isolates were purified and characterized morphologically. However, isolates with similar characteristics were discarded and only six were evaluated to determine their effect on the early growth of glutinous corn (Zea mays L.) through a pot experiment. Promising isolates were biochemically characterized to determine their classification. Corn plants inoculated with bacterial isolates were significantly taller than the control from 14 to 28 days after planting (DAE). At 28 DAE, the heights of the corn plants inoculated with LR10 (44.50cm), SC5 (43.00cm), SC4 (42.50cm), LR9 (42.33), and LB1 (40.67cm) were comparable to those inoculated with LR (39.33cm) but significantly taller than the control (35.37cm). The total biomass of corn plants inoculated with LB1 produced the heaviest (3.20g) followed by those inoculated with LR1 (2.57g) and SC4 (2.40g). Results of biochemical characterization indicate that isolate LB1 is classified as Paenibacillus polymyxa, LR1 as Bacillus subtilis, and SC4 as Bacillus subtilis.



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