Anti-angiogenic Activity of Moringa oleifera Ethanolic Leaf Extract in Chorioallantoic Membrane (CAM) Assay

Grace  V. Villanueva,; Carlos  Achondo Jr.,; Ketsara  Acctanasiri,; Shane  Agraan,; Liezel  Alingayao,; Yelena  Anzures,; Meggyrose  Apalisok,; Ederlyn  Araya,; Mary Rolhalane Rose  Arenque,; Melsergio  Arocena,; Ankit  Gupta,; Soumamitra  Dey,; Arpit  Patel,

Anti-angiogenic Activity of Moringa oleifera Ethanolic Leaf Extract in Chorioallantoic Membrane (CAM) Assay

Abstract:

Objective: This study aims to determine the possibility of using Moringa oleifera, commonly known locally as “malungay,” as an anti-angiogenic for cancer therapy. Methods: Thirty-day old fertilized duck embryos were incubated for 10 days and treated with metabolic extract of “malungay” with three different concentrations at 25%, 50% and 75%, absorbed on sterile filter paper discs. A disc containing 80% ethanol was used as a negative control and a 200 mg celecoxib + 200ml distilled water was used as a positive control. Results: Results showed no new blood vessels formation in concentrations 25%, 50% and 75% as well as in negative control. The positive control which received 2% celecoxib showed formation of new blood vessels in the membranes of four test eggs. Conclusion: The results of the study were consistent with the previous studies with the use of Moringa oleifera in the prevention of angiogenesis and treatment of inflammatory conditions such as neoplasm. Further study is needed to determine the effectiveness of the different concentration used.

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